Research
A high calcium diet containing nonfat dry milk reduces weight gain and associated adipose tissue inflammation in diet-induced obese mice when compared to high calcium alone
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* Corresponding author: Sean H Adams sean.h.adams@ars.usda.gov
- † Equal contributors
1 Department of Nutrition, University of California, One Shields Avenue, Davis, CA, USA
2 Department of Animal Science, University of California, One Shields Ave., Davis, CA 95616, USA
3 Obesity & Metabolism Research Unit, United States Department of Agriculture-Agricultural Research Service Western Human Nutrition Research Center, 430 W. Health Sciences Dr., Davis, CA 95616, USA
Nutrition & Metabolism 2012, 9:3 doi:10.1186/1743-7075-9-3
Published: 23 January 2012Additional files
Additional file 1:
Quantitative PCR primer-probe information for metabolic and inflammatory gene targets.
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Additional file 2:
Brown adipose tissue (BAT) protein content of uncoupling protein 1 (UCP1) in DIO mice fed 0.5% Ca (Control), 1.5% Ca (High-Ca), or 1.5% Ca + nonfat dry milk (High-Ca + NFDM). Values are means + SE, n = 8/treatment (arbitrary densitometry units). Treatments with different letters are significantly different (P < 0.05). UCP1 protein expression is normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein expression. Photo shows a representative Western blot result.
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Additional file 3:
Fecal weight (A), energy (B), and energy loss (C) in DIO mice fed 0.5% Ca (Control), 1.5% Ca (High-Ca), or 1.5% Ca + nonfat dry milk (High-Ca + NFDM). Values are means + SE, n = 10/treatment. Treatments with different letters are significantly different (P < 0.05).
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