Research
Transcriptome analysis of anti-fatty liver action by Campari tomato using a zebrafish diet-induced obesity model
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* Corresponding author: Toshio Tanaka tanaka@doc.medic.mie-u.ac.jp
- † Equal contributors
1 Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Mie, Japan
2 Mie University Medical Zebrafish Research Center, Mie, Japan
3 Department of Bioinformatics, Mie University Life Science Research Center, Mie, Japan
4 Department of Omics Medicine, Mie University Industrial Technology Innovation Institute, Mie, Japan
5 Department of Translational Medical Science, Mie University Graduate School of Medicine, Mie, Japan
6 Delica Foods Co Ltd, Tokyo, Japan
Nutrition & Metabolism 2011, 8:88 doi:10.1186/1743-7075-8-88
Published: 13 December 2011Abstract
Background
High dietary intake of vegetable products is beneficial against obesity and its related diseases such as dyslipidemia, nonalcoholic fatty liver disease, and cancer. We previously developed a diet-induced obesity model of zebrafish (DIO-zebrafish) that develops visceral adiposity, dyslipidemia, and liver steatosis. Zebrafish is a polyphagous animal; thus we hypothesized that DIO-zebrafish could be used for transcriptome analysis of anti-obesity effects of vegetables.
Results
Each vegetable exhibited different effects against obesity. We focused on "Campari" tomato, which suppressed increase of body weight, plasma TG, and lipid droplets in livers of DIO-zebrafish. Campari tomato decreased srebf1 mRNA by increase of foxo1 gene expression, which may depend on high contents of β-carotene in this strain.
Conclusions
Campari tomato ameliorates diet-induced obesity, especially dyslipidemia and liver steatosis via downregulation of gene expression related to lipogenesis. DIO-zebrafish can discriminate the anti-obesity effects of different strains of vegetables, and will become a powerful tool to assess outcomes and find novel mechanisms of anti-obesity effects of natural products.