Table 5

Total RNA yields obtained from mature adipocytes and stromal vascular fraction in CLA treated mice

RNA yield (μg RNA/g of epididymal depot)


Control

CLA1

CLA2

Mature adipocytes

5.5 ± 0.6a

9.8 ± 0.7b

8.8 ± 0.8b

Stromal vascular fraction

8.8 ± 0.4

6.1 ± 1.2

11.0 ± 2.5


Epididymal adipose tissue was digested by collagenase and then separated into mature adipocytes and stromal vascular fraction. RNA extracted from each fraction was quantified and referred per gram of epididymal adipose tissue weight. Data are expressed in μg RNA per g of epididymal tissue and are the means ± SEM of 7-8 mice/group. Means in a row without a common letter differ, P < 0.05 (ANOVA followed by LSD test).

Parra et al. Nutrition & Metabolism 2010 7:5   doi:10.1186/1743-7075-7-5

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