Figure 4.

TUNEL Assay (immunoflurescent labelling) and Western-blot analysis. A: C57BL/6J mice, apoptotic liver cells radiating strong green light, normal cells radiating weak green light:DBA/2J mice, little apoptotic liver cells appeared. Time-course is shown from left to right; B: Apoptosis index (percentage) in C57BL/6J and DBA/2J mice. X-axis represent time-points, y-axis represents cell apoptosis percentage. *P < 0.05 (compared to 0 week), #P < 0.05(compared to DBA/2J). C: Western blot analysis for selected proteins indicated proteins expression was also differentially changed. Proteins regulation was seen for Hspa1b, Parp and Cyp7a1.Beta-Actin served as loading control.