Effects of White Tea extract solution on triglyceride accumulation during preadipocyte/adipocyte differentiation. (A) Triglyceride accumulation and (C) cell viability of maturing preadipocytes incubated with different amounts of White Tea extract solutions (0.1%, 0.25%, 0.5% and 0.75%) is shown relative to untreated control cells set as 100%. For experiments, ten independent cell cultures were prepared both for control and White Tea extract solution incubation (n = 10). Results are depicted as mean ± SD. Significant differences are marked with an asterisk (* for p < 0.0001). (B) Images after triglyceride staining (yellow) from cell populations incubated with and without 0.5% White Tea extract solution. Scale bar: 200 μm. (D) Displayed are phase contrast images from cell populations cultivated in the absence or presence of 0.5% White Tea extract solution. Scale bar: 50 μm. For our studies we used cells up to the third passage.
Söhle et al. Nutrition & Metabolism 2009 6:20 doi:10.1186/1743-7075-6-20