Figure 1.

Expression of the genes encoding components of the ubiquitin proteasome proteolytic pathway. Muscle biopsies were taken from calves on the final day of a five day intravenous infusion of saline (control); glucose and essential amino acids (EAA). 10 μg of muscle RNA was blotted on a nylon membrane and hybridised against cDNA probes for 14-kDa E2, polyubiquitin, C2 and C8 as described under experimental procedures. A; values are means, normalised to GAPDH, +/- SEM, and represented as arbitrary units. B; representative northern blots.