Figure 4.

Pharmacology causing decreases or increases in fat metabolism is visually detectable in a quantitative fashion by using live zebrafish nile red fluorescence microscopy. Zebrafish were incubated with daily refreshing of fish water containing nile red with or without 100 μM resveratrol (R) or 10 nM troglitazone (TZD). Exposure time for all fluorescent images was set to that of untreated zebrafish (left). Bright field images are provided to show lack of overt toxicity.