Effect of curcumin on NF-kB activation. NF-kB activation was determined in the retinal homogenate by ELISA method (kit from Active Motif.) using antibody specific for p65 subunit of NF-kB. Secondary antibody conjugated to horseradish peroxidase was used to quantify the activated form spectrophotometrically. The figure represents mean ± SD of 5–6 rats each in of the 3 groups. *P < 0.05 compared with normal or diabetes + curcumin groups.
Kowluru and Kanwar Nutrition & Metabolism 2007 4:8 doi:10.1186/1743-7075-4-8