Figure 4.

The expression of UCP2 mRNA (A) and UCP2 protein expression (B and C) in WT islets. UCP2 expression was determined by quantitative RT-PCR in WT islets after 24 h incubation with either BSA (open bars), OA (horizontal bars) or PA (closed bars) and also in those pre-treated with siPPARα then cultured with BSA (vertical bars), OA (left-hatched bars) and PA (right-hatched bars). The same treatment and incubation time was applied to detect the UCP2 protein expression by Western blotting. Treatment of WT islets with PA for 24 h significantly increased UCP2 mRNA and protein expression (*P < 0.05 vs BSA or siBSA). Data are presented as means ± SE, N = 5 or greater for each group.