Expression of β-OHBDH and SCOT in B6 and SCID mouse brains and in CT-2A and U87 brain tumors. (A) RT-PCR was used to detect mRNA levels of β-OHBDH, SCOT, and β-actin as described in methods. Expression was evaluated in normal appearing B6 brain tissue (B6-B) contralateral to the CT-2A tumor, and in normal appearing SCID brain tissue (SCID-B) contralateral to the U87 tumor. (B) The ratio (relative intensity) of β-OHBDH and SCOT expression to β-actin expression. The values are expressed as the mean of three-four independent samples with error bars representing SEM. The asterisks indicate that the ratio was significantly less in the tumor tissue (solid bars) than in the normal brain tissue (open bars) at P < 0.01.
Zhou et al. Nutrition & Metabolism 2007 4:5 doi:10.1186/1743-7075-4-5