Influence of diet on vascularity in the CT-2A (A and B) and the U87 (C) brain tumors grown orthotopically in B6 or SCID mice, respectively. Vessels were stained with the Factor VIII antibody and each stained section was representative of the entire tumor. For quantitative analysis of vessels in the CT-2A tumor, microvessel density was expressed as the number of vessels/200 × high power field (hpf). The values are expressed as the mean of three independent samples with error bars representing SEM. The asterisks indicate that the number of vessels/hpf was significantly less in the KC-R group than in the SD-UR group at P < 0.05. The scale bar represents 250 μm.
Zhou et al. Nutrition & Metabolism 2007 4:5 doi:10.1186/1743-7075-4-5