Table 5

Mean number of copies per cell of the mRNAs for the selected genes in liver and adipose tissue of overweight male rats subjected to food restriction

Gene name

Control

Restricted feeding

Cycles

cpc

Cycles

cpc

Liver

60S acidic ribosomal protein P0 (62 bp)

20.1 ± 0.1

1157 ± 50

20.1 ± 0.1

801 ± 38*

Cyclophilin A

19.4 ± 0.1

1261 ± 44

19.6 ± 0.1

855 ± 13*

Carbohydrate-responsive element-binding protein

22.8 ± 0.1

94 ± 6

23.3 ± 0.1*

54 ± 3*

Acetyl-CoA carboxylase 1

23.4 ± 0.2

50 ± 3

24.6 ± 0.1*

19 ± 1*

Insulin receptor substrate 1

25.0 ± 0.2

13 ± 1

24.9 ± 0.2

12 ± 1

Retroperitoneal WAT

60S acidic ribosomal protein P0 (62 bp)

19.7 ± 0.1

160 ± 18

19.7 ± 0.1

191 ± 29

Cyclophilin A

19.1 ± 0.1

193 ± 30

19.4 ± 0.1

149 ± 7

Carbohydrate-responsive element-binding protein

24.4 ± 0.3

4.3 ± 0.6

26.2 ± 0.4*

1.0 ± 0.2*

Type II iodothyronine deiodinase

27.0 ± 0.1

0.63 ± 0.10

28.1 ± 0.2*

0.29 ± 0.03*

Acetyl-CoA carboxylase 1

23.0 ± 0.2

7.6 ± 1.8

23.5 ± 0.6

6.8 ± 2.2

Insulin receptor substrate 1

26.6 ± 0.2

0.46 ± 0.05

27.9 ± 0.4*

0.15 ± 0.04*

The data are the mean ± sem of 7 different animals; cpc = copies of the corresponding mRNA per cell. Statistical significance of the differences between groups (Student's t test) * = P < 0.05.

Romero et al. Nutrition & Metabolism 2007 4:26   doi:10.1186/1743-7075-4-26