Table 1

Steroid production by control (non-transfected) and SR-BI transfected Y1-BS1 mouse adrenocortical cells

Results are Mean ± SE of four separate experiments.

Cultures of Y1-BS1 cells were transfected with rSR-BI-V5-pcDNA6.1 (or vector control) constructs for 48 h. Twenty four hours after transfection, some cultures were treated with Bt₂cAMP (2.5 mM) for an additional 24 h. Subsequently, cells were incubated for 5 h in the absence (basal) or presence of Bt₂cAMP (2.5 mM) ± hHDL₃ (500 μg protein/ml) as indicated. Following incubation, a suitable aliquot of the medium from each sample was collected, and steroids were extracted from the medium using methylene chloride and quantified by fluorescence in 65% sulfuric acid-35% ethanol using corticosterone as a standard.

^¶ p = 0.0023 vs Bt₂cAMP + hHDL₃-treated control cells

Reaven et al. Nutrition & Metabolism 2006 3:43   doi:10.1186/1743-7075-3-43