Table 1

Effects of AICAR treatment on fatty acid composition (weight %) of adipose tissue in normal and diet-induced obese mice

Group/Fatty acid

14:0

16:0

18:0

18:2

STD

0.89 ± 0.02

18.6 ± 0.1

1.9 ± 0.06

27.1 ± 0.35

STD+A500

0.9 ± 0.01

22.2 ± 0.21

3.0 ± 0.15

28.9 ± 0.81

DIO

1.3 ± 0.2

26.6 ± 0.21*

3.6 ± 0.15*

15.9 ± 1.3*

DIO+A100

0.95 ± 0.03

24.3 ± 0.38

4.4 ± 0.06

28.7 ± 0.86

DIO+A500

1.1 ± 0.1

23.3 ± 0.26**

2.5 ± 0.21**

22.2 ± 0.7**

Adipose tissue was isolated and prepared as described in Methods. The lipids were extracted in chloroform-methanol using Folch partition. Fatty acid methyl ester was prepared with methanolic-HCl, purified by TLC and analyzed by GC as described in our earlier publication [29]. Fatty acids showing significant alteration in composition are included and their peaks were identified using known standards. Values are expressed as mean ± SD for 3 separate sets of experiments. *p < 0.001 vs STD, **p < 0.001 vs DIO.

Giri et al. Nutrition & Metabolism 2006 3:31   doi:10.1186/1743-7075-3-31