Figure 7.

Effect of AICAR on adipocyte size and total DNA content in AICAR treated STD and DIO mice. Paraffin-embedded sections of epididymal white adipose tissue from AICAR treated STD and DIO mice were stained with hematoxylin and eosin (ai). Average size of adipocytes measured in 10 different fields is shown at right (aii). ***p < 0.001; NS: not significant as compared to STD mice group. $ p < 0.001, as compared to DIO vehicle treated group. NS: not significant as compared to STD mice group. White adipose tissue from these treated STD and DIO mice were digested with proteinase K and DNA was extracted with phenol:chloroform. DNA content was measured by spectrophotometer (260 nm) (b). NS: not significant as compared to STD mice group. $: not significant as compared to DIO vehicle treated group.