Figure 12.

AICAR induced the expression of PGC1α in white adipose tissue in DIO treated mice. Total protein from epididymal fat content from 6 weeks AICAR treated STD and DIO treated mice was isolated and immunoblot analysis for PGC1α was examined (a i). Densitometry values for PGC1α were normalized with β actin and plotted as a bar graph (a ii). The expression of PGC1α was quantified by qPCR (b). The relative qPCR values were normalized with respect to the 18S rRNA expression level. Values are mean ± S.D. for three mice. *** p < 0.001; ** p < 0.01 as compared to STD mice group. $ p < 0.001, as compared to DIO vehicle treated group. The differentiating 3T3-L1 cells were treated with different concentrations of AICAR (from 0.1 to 1.0 mM) and proteins were isolated at various time periods (from 0 to 9 days). Immunoblot analysis for PGC1α and β actin was examined as described in Materials and Methods (c).