|
Dependence of binding kinetics on pH and ionic strength as measured by SPR |
|||||
| Immobilized reagent |
pH |
NaCl (mmol/L) |
kon (104 M-1s-1) |
koff (10-4 s-1) |
KD (nmol/L) |
|
|
|||||
| HoloTC |
6.0 |
0 |
10 |
1 |
1 |
| 7.4 |
0 |
8 |
0.4 |
0.5 |
|
| 7.4 |
300 |
7 |
0.6 |
0.9 |
|
| 8.0 |
0 |
7 |
0.2 |
0.3 |
|
| MAb 3C4 |
6.0 |
0 |
50 |
4 |
0.8 |
| 6.0 |
300 |
0.3 |
35 |
1340 |
|
| 7.4 |
0 |
3 |
14 |
46 |
|
| 7.4 |
300 |
0.3 |
41 |
1380 |
|
| 8.0 |
0 |
7 |
19 |
27 |
|
Orning et al. Nutrition & Metabolism 2006 3:3 doi:10.1186/1743-7075-3-3 |
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