Table 2

The conversion of glucose, acetate and glutamate to CO2 and lipid by liver slices from PEPCK-C-/- and control mice.

CO2
 Total Lipid
Substrates
 PEPCK-C+/+
 (n)
 PEPCK-C-/-
 (n)
 PEPCK-C+/+
 (n)
 PEPCK-C-/-
 (n)
Glucose-U-14C
 0.92 ± 0.26
 (8)
 0.30 ± 0.04
 (5)
 0.153 ± 0.009
 (3)
 0.063 ± 0.002
 (2)
Acetate-2-14C
 12.36 ± 0.88
 (4)
 2.72 ± 0.20
 (5)
 0.097 ± 0.018
 (7)
 0.002 ± 0.000
 (2)
Glutamate-5-14C
 5.15 ± 0.65
 (2)
 1.75 ± 0.65
 (2)
 1.778 ± 0.091
 (4)
 0.983 ± 0.098
 (3)

The values are expressed as μmoles of substrate converted to product/hour/g liver and represent the mean ± SE of the mean for the number of determinations shown in parenthesis. Livers slices from two-day-old mice were incubated for 2 h in Krebs Ringer bicarbonate buffer, pH 7.4, containing substrates at the concentrations outlined in the Methods, and the rates of 14CO2 production and 14C incorporation into total lipid determined. The radioactivity was quantified using a liquid scintillation spectrometer.

Hakimi et al. Nutrition & Metabolism 2005 2:33   doi:10.1186/1743-7075-2-33