Figure 2.

Immunocytochemical staining of OPG in bone from Mg deficient and control animals. A. Represents animals on a 25% NR diet and B. the control group. Note the minimal staining of osteoclasts (solid arrows) and osteoblasts (open arrows) in A. while positive staining is observed the control animals (B). C. Represents animals on a 50% NR diet and D. the control group. Again, as observed in C., Mg deficient animals have minimal staining for OPG in osteoclasts (solid arrows) and osteoblasts (open arrows), while much more intense staining is observed in the control animals (D).