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Open Access Highly Accessed Research

Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

Abeer A Ahmed1, Kayode A Balogun1, Natalia V Bykova23 and Sukhinder K Cheema1*

Author Affiliations

1 Department of Biochemistry, Memorial University of Newfoundland, St. John’s, NL, A1B 3X9, Canada

2 Department of Biology, Memorial University of Newfoundland, St. John’s, NL, Canada

3 Current address: Agriculture and AgriFood Canada, Winnipeg, MB, Canada

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Nutrition & Metabolism 2014, 11:6  doi:10.1186/1743-7075-11-6

Published: 17 January 2014

Abstract

Background

Omega-3 polyunsaturated fatty acids (n-3 PUFA) have been shown to alleviate the symptoms of metabolic disorders, such as heart disease, diabetes, obesity and insulin resistance. Several putative mechanisms by which n-3 PUFA elicit beneficial health effects have been proposed; however, there is still a shortage of knowledge on the proteins and pathways that are regulated by n-3 PUFA.

Methods

Using two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, we investigated the effects of diets high or low in n-3 PUFA on hepatic proteomic profile of C57BL/6 mice.

Results

The findings show for the first time that high dietary n-3 PUFA reduced the expression of regucalcin, adenosine kinase and aldehyde dehydrogenase. On the other hand, diets high in n-3 PUFA increased the expression of apolipoprotein A-I, S-adenosylmethionine synthase, fructose-1, 6-bisphosphatase, ketohexokinase, malate dehydrogenase, GTP-specific succinyl CoA synthase, ornithine aminotransferase and protein disulfide isomerase-A3.

Conclusions

Our findings revealed for the first time that n-3 PUFA causes alterations in several novel functional proteins involved in regulating lipid, carbohydrate, one-carbon, citric acid cycle and protein metabolism, suggesting integrated regulation of metabolic pathways. These novel proteins are potential targets to develop therapeutic strategies against metabolic disorders.