Gene expression in liver. The graphs showing the relative mRNA expression of genes coding for selected proteins in “Peroxisomal pathways”, “Mitochondrial pathways”, “Mevalonate pathway” and “Nuclear transcription factors” are generated from the TLDA array described in Materials and Methods. The bars show the median and range of the relative expression in the control group (HF, dark grey bars), the FO group (FO, light grey bars) and the KO group (KO, medium gray bars). Values are shown in relation to the expression of respective gene in one individual of the HF group (n = 4 per group). The graph showing “Additional metabolic pathways” was generated by real time PCR on selected genes as described in Material and Methods with the bars showing the median and the range of the relative mRNA expression in relation to one individual (set to 1) in the HF group. HF; dark grey bars (n = 6), FO; light grey bars (n = 5) KO; medium grey bars (n = 6). Significance by the Kruskal-Wallis test is shown to the right in each graph (K-W), and a star close to the bars show significance by the Dunn post hoc analysis (* = p < 0.05, # = p < 0.01 for the post hoc test).
Tillander et al. Nutrition & Metabolism 2014 11:20 doi:10.1186/1743-7075-11-20