Table 2

Effect of MTX and glutamine on microscopic intestinal parameters, cell proliferation and apoptosis
Parameters/Groups CONTR CONTR-GLN MTX MTX-GLN
Villus height (μm)
Jejunum 461 ± 16 460 ± 40 246 ± 11* 281 ± 14*†
Ileum 304 ± 26 294 ± 30 192 ± 17* 255 ± 11*†
Crypt depth (μm)
Jejunum 184 ± 7 148 ± 18 105 ± 8* 138 ± 10*†
Ileum 157 ± 7 131 ± 9 102 ± 3* 136 ± 13*†
Cell proliferation (BrdU positive cells/10 crypts)
Jejunum 158 ± 9 163 ± 13 132 ± 4* 147 ± 5†
Ileum 165 ± 14 189 ± 25 129 ± 4* 152 ± 5†
Cell apoptosis (apoptotic cells/10 villi)
Jejunum 0.7 ± 0.2 0.9 ± 0.26 1.45 ± 0.23* 1.23 ± 0.23
Ileum 0.3 ± 0.05 0.5 ± 0.14 1.44 ± 0.13* 0.85 ± 0.26*†

CONTR-control, GLN-glutamine, MTX-methotrexate.

*P < 0.05 vs control, †P < 0.05 MTX-GLN vs MTX.

Sukhotnik et al.

Sukhotnik et al. Nutrition & Metabolism 2014 11:17   doi:10.1186/1743-7075-11-17

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