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Coacervate whey protein improves inflammatory milieu in mice fed with high-fat diet

Mayara Franzoi Moreno1*, Gabriel Inácio de Morais Honorato de Souza1, Ana Claudia Losinskas Hachul1, Bruno dos Santos1, Marcos Hiromu Okuda1, Nelson Inácio Pinto Neto1, Valter Tadeu Boldarine1, Elisa Esposito2, Eliane Beraldi Ribeiro1, Claudia Maria da Penha Oller do Nascimento1, Aline de Piano Ganen1 and Lila Missae Oyama1

Author Affiliations

1 Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, São Paulo, SP, Brazil

2 Instituto de Ciências e Tecnologia da Universidade Federal de São Paulo, São José dos Campos, SP, Brazil

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Nutrition & Metabolism 2014, 11:15  doi:10.1186/1743-7075-11-15

Published: 28 March 2014



Functional foods with bioactive properties may help in treat obesity, as they can lead to a decreased risks of inflammatory diseases. The aim of this study was to investigate the effects of chitosan coacervate whey protein on the proinflammatory processes in mice fed with high-fat diet.


Mice were divided into two groups receiving either a normolipidic or high-fat diet; the animals in each of the two diet groups were given a diet supplement of either coacervate (gavage, 36 mg protein/kg of body weight) or tap water for four weeks [groups: normolipidic diet plus water (C); normolipidic diet and coacervate (CC); high-fat diet and water (H); and high-fat diet and coacervate (HC)].


The high-fat diet promoted inflammation, possibly by decreased adiponectin/sum of adipose tissues ratio and increased phosphorylation of NF-κB p50. In HC we observed a positive correlation between IL-10 and TNF-α in mesenteric adipose tissue, retroperitoneal adipose tissue and liver tissue. We also observed a positive correlation between lipopolisaccharide with IL-10 in the liver tissue.


High-fat diet treatment promoted metabolic alterations and inflammation, and chitosan coacervate whey protein modulated inflammatory milieu.

Mesenteric adipose tissue; Cytokines; IL-10