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Effects of oral adenosine-5′-triphosphate supplementation on athletic performance, skeletal muscle hypertrophy and recovery in resistance-trained men

Jacob M Wilson1*, Jordan M Joy1, Ryan P Lowery1, Michael D Roberts2, Christopher M Lockwood3, Anssi H Manninen4, John C Fuller5, Eduardo O De Souza6, Shawn M Baier5, Stephanie MC Wilson7 and John A Rathmacher58

Author Affiliations

1 Department of Health Sciences and Human Performance, The University of Tampa, Tampa FL, USA

2 Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA

3 AP Nutrition, LLC, Missoula MT, USA

4 Metabolia Oulu, Oulu, Finland

5 Metabolic Technologies Inc., Iowa State University Research Park, Ames, IA, USA

6 Laboratory of Neuromuscular Adaptations to Strength Training, School of Physical Education and Sport, University of São Paulo, São Paulo, Brazil

7 Department of Nutrition, IMG Academy, Bradenton, FL, USA

8 Department of Animal Science, Iowa State University, Ames, IA, USA

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Nutrition & Metabolism 2013, 10:57  doi:10.1186/1743-7075-10-57

Published: 22 September 2013



Currently, there is a lack of studies examining the effects of adenosine-5′-triphosphate (ATP) supplementation utilizing a long-term, periodized resistance-training program (RT) in resistance-trained populations. Therefore, we investigated the effects of 12 weeks of 400 mg per day of oral ATP on muscular adaptations in trained individuals. We also sought to determine the effects of ATP on muscle protein breakdown, cortisol, and performance during an overreaching cycle.


The study was a 3-phase randomized, double-blind, and placebo- and diet-controlled intervention. Phase 1 was a periodized resistance-training program. Phase 2 consisted of a two week overreaching cycle in which volume and frequency were increased followed by a 2-week taper (Phase 3). Muscle mass, strength, and power were examined at weeks 0, 4, 8, and 12 to assess the chronic effects of ATP; assessment performance variables also occurred at the end of weeks 9 and 10, corresponding to the mid and endpoints of the overreaching cycle.


There were time (p < 0.001), and group x time effects for increased total body strength (+55.3 ± 6.0 kg ATP vs. + 22.4 ± 7.1 kg placebo, p < 0.001); increased vertical jump power (+ 796 ± 75 ATP vs. 614 ± 52 watts placebo, p < 0.001); and greater ultrasound determined muscle thickness (+4.9 ± 1.0 ATP vs. (2.5 ± 0.6 mm placebo, p < 0.02) with ATP supplementation. During the overreaching cycle, there were group x time effects for strength and power, which decreased to a greater extent in the placebo group. Protein breakdown was also lower in the ATP group.


Our results suggest oral ATP supplementation may enhance muscular adaptations following 12-weeks of resistance training, and prevent decrements in performance following overreaching. No statistically or clinically significant changes in blood chemistry or hematology were observed.

Trial registration NCT01508338

Adenosine triphosphate; Exercise performance; Power; Strength; Muscle hypertrophy; Sports nutrition